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Synaptic linkage analysis between the PL-mPFC and the NAc core. ( A ) The schematic diagram of the electrophysiological experimental design. ( B ) The schematic representation of the viral injection site in the PL-mPFC (left) and PL-NAc virus projection (right) (scale bar: 1000 μm). ( C ) Action potentials were recorded in neurons expressing ChR2-eYFP in the PL-mPFC in current-clamp mode (blue light: 465 nm, 43.7 mW, 5 ms duration) at 5 Hz in order to identify the virus function (blue vertical lines represent light <t>stimulation</t> pulses). ( D ) The eEPSC was recorded in each group. The eEPSC was completely blocked by TTX (1 μM) and recovered by consequent 4-AP (100 μM) (ACSF vs TTX: P < 0.01; TTX vs TTX + 4-AP: P < 0.01). ( E ) The typical eEPSC trajectory diagrams of three groups. The colors displayed are as follows: ACSF group in black, TTX group in red, and TTX + 4-AP group in blue. ** P < 0.01.
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blue light stimulation  (CoolLED Inc)
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Synaptic linkage analysis between the PL-mPFC and the NAc core. ( A ) The schematic diagram of the electrophysiological experimental design. ( B ) The schematic representation of the viral injection site in the PL-mPFC (left) and PL-NAc virus projection (right) (scale bar: 1000 μm). ( C ) Action potentials were recorded in neurons expressing ChR2-eYFP in the PL-mPFC in current-clamp mode (blue light: 465 nm, 43.7 mW, 5 ms duration) at 5 Hz in order to identify the virus function (blue vertical lines represent light <t>stimulation</t> pulses). ( D ) The eEPSC was recorded in each group. The eEPSC was completely blocked by TTX (1 μM) and recovered by consequent 4-AP (100 μM) (ACSF vs TTX: P < 0.01; TTX vs TTX + 4-AP: P < 0.01). ( E ) The typical eEPSC trajectory diagrams of three groups. The colors displayed are as follows: ACSF group in black, TTX group in red, and TTX + 4-AP group in blue. ** P < 0.01.
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https://www.bioz.com/result/blue light stimulation/product/CoolLED Inc
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Synaptic linkage analysis between the PL-mPFC and the NAc core. ( A ) The schematic diagram of the electrophysiological experimental design. ( B ) The schematic representation of the viral injection site in the PL-mPFC (left) and PL-NAc virus projection (right) (scale bar: 1000 μm). ( C ) Action potentials were recorded in neurons expressing ChR2-eYFP in the PL-mPFC in current-clamp mode (blue light: 465 nm, 43.7 mW, 5 ms duration) at 5 Hz in order to identify the virus function (blue vertical lines represent light <t>stimulation</t> pulses). ( D ) The eEPSC was recorded in each group. The eEPSC was completely blocked by TTX (1 μM) and recovered by consequent 4-AP (100 μM) (ACSF vs TTX: P < 0.01; TTX vs TTX + 4-AP: P < 0.01). ( E ) The typical eEPSC trajectory diagrams of three groups. The colors displayed are as follows: ACSF group in black, TTX group in red, and TTX + 4-AP group in blue. ** P < 0.01.
Fiber Optic Light Stimulating Device 465 Nm Blue Led, supplied by A-M Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fiber-optic light stimulating device with a 465-nm blue led  (A-M Systems)
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A-M Systems fiber-optic light stimulating device with a 465-nm blue led
Synaptic linkage analysis between the PL-mPFC and the NAc core. ( A ) The schematic diagram of the electrophysiological experimental design. ( B ) The schematic representation of the viral injection site in the PL-mPFC (left) and PL-NAc virus projection (right) (scale bar: 1000 μm). ( C ) Action potentials were recorded in neurons expressing ChR2-eYFP in the PL-mPFC in current-clamp mode (blue light: 465 nm, 43.7 mW, 5 ms duration) at 5 Hz in order to identify the virus function (blue vertical lines represent light <t>stimulation</t> pulses). ( D ) The eEPSC was recorded in each group. The eEPSC was completely blocked by TTX (1 μM) and recovered by consequent 4-AP (100 μM) (ACSF vs TTX: P < 0.01; TTX vs TTX + 4-AP: P < 0.01). ( E ) The typical eEPSC trajectory diagrams of three groups. The colors displayed are as follows: ACSF group in black, TTX group in red, and TTX + 4-AP group in blue. ** P < 0.01.
Fiber Optic Light Stimulating Device With A 465 Nm Blue Led, supplied by A-M Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Synaptic linkage analysis between the PL-mPFC and the NAc core. ( A ) The schematic diagram of the electrophysiological experimental design. ( B ) The schematic representation of the viral injection site in the PL-mPFC (left) and PL-NAc virus projection (right) (scale bar: 1000 μm). ( C ) Action potentials were recorded in neurons expressing ChR2-eYFP in the PL-mPFC in current-clamp mode (blue light: 465 nm, 43.7 mW, 5 ms duration) at 5 Hz in order to identify the virus function (blue vertical lines represent light stimulation pulses). ( D ) The eEPSC was recorded in each group. The eEPSC was completely blocked by TTX (1 μM) and recovered by consequent 4-AP (100 μM) (ACSF vs TTX: P < 0.01; TTX vs TTX + 4-AP: P < 0.01). ( E ) The typical eEPSC trajectory diagrams of three groups. The colors displayed are as follows: ACSF group in black, TTX group in red, and TTX + 4-AP group in blue. ** P < 0.01.

Journal: Journal of Pain Research

Article Title: Restoration of the Activity of the Prefrontal Cortex to the Nucleus Accumbens Core Pathway Relieves Fentanyl-Induced Hyperalgesia in Male Rats

doi: 10.2147/JPR.S442765

Figure Lengend Snippet: Synaptic linkage analysis between the PL-mPFC and the NAc core. ( A ) The schematic diagram of the electrophysiological experimental design. ( B ) The schematic representation of the viral injection site in the PL-mPFC (left) and PL-NAc virus projection (right) (scale bar: 1000 μm). ( C ) Action potentials were recorded in neurons expressing ChR2-eYFP in the PL-mPFC in current-clamp mode (blue light: 465 nm, 43.7 mW, 5 ms duration) at 5 Hz in order to identify the virus function (blue vertical lines represent light stimulation pulses). ( D ) The eEPSC was recorded in each group. The eEPSC was completely blocked by TTX (1 μM) and recovered by consequent 4-AP (100 μM) (ACSF vs TTX: P < 0.01; TTX vs TTX + 4-AP: P < 0.01). ( E ) The typical eEPSC trajectory diagrams of three groups. The colors displayed are as follows: ACSF group in black, TTX group in red, and TTX + 4-AP group in blue. ** P < 0.01.

Article Snippet: To record light-evoked excitatory postsynaptic currents (eEPSC) and light-excited inhibitory postsynaptic currents (eIPSC), 5 mM QX-314 (MCE, New Jersey, USA) was added to the internal solution, and blue light stimulation was provided using a 40 × water-immersion objective lens (Eclipse FN1, Nikon) for 2 ms to activate channelrhodopsin-2 (ChR2) (pE-300 white, CoolLED co.,ltd, Andover, UK). eEPSC and eIPSC were recorded in voltage-clamp mode at −70 mV and −30 mV, respectively.

Techniques: Injection, Virus, Expressing